Molecular cloning : a laboratory manual

Abstract

Volumen 1: Chapter 1. Plasmids and Their Usefulness in Molecular Cloning -- Chapter 2. Bacteriophage and lts Vectors -- Chapter 3. Working with Bacteriophage M13 Vectors -- Chapter 4. Working with High-capacity Vectors -- Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose -- Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA -- Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells -- Volumen 2. Chapter 8. In Vitro Amplification of DNA by the Polymerase Chain Reaction -- Chapter 9. Preparation of Radiolabeled DNA and RNA Probes -- Chapter 10. Working with Synthetic Oligonucleotide Probes -- Chapter 11. Preparation of cDNA Libraries and Gene ldentification -- Chapter 12. DNA Sequencing -- Chapter 13. Mutagenesis -- Chapter 14. Screening Expression Libraries -- Volumen 3. Chapter 15. Expression of Cloned Genes in Eschcrichia coli -- Chapter 16. lntroducing Cloned Genes into Cultured Mammalian Cells -- Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells -- Chapter 18. Protein lnteraction Technologies